LOCUS       pTCF\20       6178 bp    DNA   circular            19-FEB-2003
DEFINITION  Kd-mut cut with NdeI/BamHI, ligated into similarly cut JA 33.
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     CDS             3733..4545
                     /vntifkey="4"
                     /label=Kan
     rep_origin      3024..3024
                     /vntifkey="33"
                     /label=pBR322\origin
     CDS             511..1590
                     /vntifkey="4"
                     /label=lacI
     CDS             complement(5..37)
                     /vntifkey="4"
                     /label=T7\Tag
     promoter        complement(108..124)
                     /vntifkey="30"
                     /label=T7\Promoter
     CDS             81..106
                     /vntifkey="4"
                     /label=Lac\Operator
     CDS             complement(5340..6176)
                     /vntifkey="4"
                     /label=Kd\Sol
     CDS             5286..5339
                     /vntifkey="4"
                     /label=BSP41\sequence
BASE COUNT     1422 a      1655 c      1659 g      1442 t 
ORIGIN
        1 tatgacccat ttgctgtcca ccagtcatgc tagccatggt atatctcctt cttaaagtta 
       61 aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt 
      121 attaatttcg cgggatcgag atctcgatcc tctacgccgg acgcatcgtg gccggcatca 
      181 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc 
      241 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg 
      301 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc 
      361 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc 
      421 gtcgagatcc cggacaccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc 
      481 ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca 
      541 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt 
      601 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac 
      661 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt 
      721 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg 
      781 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg 
      841 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac 
      901 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc 
      961 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc 
     1021 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt 
     1081 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt 
     1141 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg 
     1201 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg 
     1261 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta 
     1321 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa 
     1381 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc 
     1441 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg 
     1501 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 
     1561 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct 
     1621 cactcattag gcaccgggat ctcgaccgat gcccttgaga gccttcaacc cagtcagctc 
     1681 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat 
     1741 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg 
     1801 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc 
     1861 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc 
     1921 cggcatggcg gccccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg 
     1981 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag 
     2041 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg 
     2101 tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg 
     2161 catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg 
     2221 gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc 
     2281 ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct 
     2341 ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc 
     2401 agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt 
     2461 aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc 
     2521 gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg 
     2581 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 
     2641 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc 
     2701 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag 
     2761 cagattgtac tgagagtgca ccatatatgc ggtgtgaaat accgcacaga tgcgtaagga 
     2821 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 
     2881 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 
     2941 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 
     3001 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 
     3061 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 
     3121 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 
     3181 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 
     3241 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 
     3301 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 
     3361 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 
     3421 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 
     3481 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 
     3541 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 
     3601 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 
     3661 actcacgtta agggattttg gtcatgaaca ataaaactgt ctgcttacat aaacagtaat 
     3721 acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctctaggcc gcgattaaat 
     3781 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca 
     3841 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat 
     3901 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg 
     3961 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta 
     4021 ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca 
     4081 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt 
     4141 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg 
     4201 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa 
     4261 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat 
     4321 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat 
     4381 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc 
     4441 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct 
     4501 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaaga attaattcat 
     4561 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 
     4621 tccccgaaaa gtgccacctg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 
     4681 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 
     4741 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 
     4801 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 
     4861 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 
     4921 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 
     4981 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 
     5041 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca 
     5101 ttcgccaatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 
     5161 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 
     5221 cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca 
     5281 agcttttaac gatgattcca caccattttc tgtgcatcca gaatatgatg cagggatccg 
     5341 gatggaggaa gttcccatct cagggtgaga ggctcaggca gccccttatg gtgcacatgg 
     5401 catgtgtaat tctgctcctt cccaagaggc accaccacag ctgcccactt ctggaaggtt 
     5461 ccatcccctg caggcctggt ctctacaagc tccatgtcct gggtcaggtc ctccccattc 
     5521 aactgccagg tcagggtgat atcagcaggg tagaagccca gggcccagca cctcagggtg 
     5581 acatcaactt gagatctggg gtgataggtc acatgggcct ttggggaatc tgtgcgcagc 
     5641 agcgtctcat tcccgagctc caggtatctg cggagccact ccacgcactc gccctctagg 
     5701 taggccctgt aatactctgc atcaccagcc tgctcccact tgcgtctggt gatcagcgcc 
     5761 gccgtgtccg ccgccgtcca cgttttcagg tcttcgttca gggcgatgta atcgcggccg 
     5821 tcgtaggcga actgatggta cccgcggagg aggcgccagt ccgaccccac gtcacagccg 
     5881 aacatccgct ggaacgtgtg agagccgccc ttgctctggt tgtagtatct ctgtgcggtc 
     5941 ctcaggctca ctcggaacca ctgctcatcg ctcttggctc tctgtgtctg ctcctcccaa 
     6001 tactccggcc cctcctgctc catccacggc gcccgcggct caaatctcgg attatccgcg 
     6061 tcgctgtcga agcgcacgaa ctgcgtgtcg tccacgtagc cgacagcgat gaaccggggc 
     6121 tccccgaggc cgggccggga cacggcggtg acgaaatatc taagagaatg tggaccca 
//