LOCUS       pTCF\23       6149 bp    DNA   circular            19-FEB-2003
DEFINITION  H-2Dq cut with NheI/BamHI,  ligated into similarly cut JA 60.
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     CDS             complement(6..842)
                     /vntifkey="4"
                     /label=H-2Dq
     CDS             complement(5456..5911)
                     /vntifkey="4"
                     /label=f1\origin
     CDS             4548..5360
                     /vntifkey="4"
                     /label=Kan
     promoter        complement(923..939)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      3839..3839
                     /vntifkey="33"
                     /label=pBR322\origin
     CDS             1326..2405
                     /vntifkey="4"
                     /label=lacI
     CDS             complement(6062..6079)
                     /vntifkey="4"
                     /label=His\Tag
     CDS             complement(6101..6148)
                     /vntifkey="4"
                     /label=BSP\Sequence
BASE COUNT     1408 a      1654 c      1655 g      1432 t 
ORIGIN
        1 gatccggacg gaggaggctc ccatctcagg gtgaggggct caggcagccc ctcatggtac 
       61 acacggcatg tgtaattctg ctccttccca agaggcacca ccacagatgc ccacttctgg 
      121 aaggttccat cccctgcagg cctggtctcc acaagctcca tgtcctgggt cagctcctcc 
      181 ccattcaact gccaggtcag ggtgatgtca gcagggtaga agcccagggc ccagcacctc 
      241 agggtgactt cacctttaga tctggggtga tgggtcacat gtgcctttgg ggaatctgtg 
      301 cgcagcagcg tcgcgttccc gttcttcagg tatctgtgga gccactccac gcactcgccc 
      361 tccaggtagg ccttgtaatg ctctgcagca ccaccctgct cccacttgcg tcgggtgatc 
      421 tgcgccgcca tgtccgccgc cgtccacgtt ttcaggtctt cgttcagggc gatgtaatcg 
      481 aggccgtcgt aggcgaactg ctcgtacccg cggaggaggc gcccgtccaa ccccacgtca 
      541 cagccgtaca tccgctggat cgtgtgagag ccgcccgcgc tctggttgta gtagcgcagc 
      601 gcggtcctca ggctcactcg gaaccactgc tcattgtcct tggcgatctg cgtgatccgc 
      661 tcccaatact ccggcccctc ctgctccatc cacggcgccc gcggctcata tctcggattc 
      721 tccgcgtcgc tgtcgaagcg cacgaactcc ttgttgtcca catagccgac agagatgtac 
      781 cggggctccc cgaggccggg ccgggacacg gcggtctcga aataacgcat agaatgtgga 
      841 ccgctagcca tatgtatatc tccttcttaa agttaaacaa aattatttct agaggggaat 
      901 tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatctc 
      961 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg 
     1021 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag 
     1081 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat 
     1141 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg 
     1201 ctgcttccta atgcaggagt cgcataaggg agagcgtcga gatcccggac accatcgaat 
     1261 ggcgcaaaac ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg 
     1321 tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga 
     1381 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg 
     1441 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca 
     1501 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa 
     1561 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg 
     1621 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg 
     1681 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg 
     1741 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta 
     1801 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc 
     1861 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg 
     1921 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg 
     1981 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc 
     2041 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg 
     2101 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca 
     2161 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa 
     2221 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt 
     2281 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc 
     2341 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 
     2401 ggcagtgagc gcaacgcaat taatgtaagt tagctcactc attaggcacc gggatctcga 
     2461 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact 
     2521 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca 
     2581 gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg 
     2641 tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc 
     2701 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggcccc acgggtgcgc 
     2761 atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag 
     2821 cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg 
     2881 acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg 
     2941 aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc 
     3001 tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc 
     3061 tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca 
     3121 tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc 
     3181 cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga aaaaaccgcc 
     3241 cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag 
     3301 ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt 
     3361 taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc 
     3421 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc 
     3481 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc 
     3541 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata 
     3601 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc 
     3661 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 
     3721 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 
     3781 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 
     3841 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 
     3901 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 
     3961 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 
     4021 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 
     4081 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 
     4141 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 
     4201 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 
     4261 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 
     4321 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 
     4381 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 
     4441 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 
     4501 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc 
     4561 aacgggaaac gtcttgctct aggccgcgat taaattccaa catggatgct gatttatatg 
     4621 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg 
     4681 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg 
     4741 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca 
     4801 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccgggaaaa 
     4861 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg 
     4921 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc 
     4981 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg 
     5041 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac 
     5101 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta 
     5161 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc 
     5221 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga 
     5281 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt 
     5341 tgatgctcga tgagtttttc taagaattaa ttcatgagcg gatacatatt tgaatgtatt 
     5401 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaaatt 
     5461 gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt 
     5521 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg 
     5581 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc 
     5641 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca 
     5701 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga 
     5761 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa 
     5821 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc 
     5881 gccgcgctta atgcgccgct acagggcgcg tcccattcgc caatccggat atagttcctc 
     5941 ctttcagcaa aaaacccctc aagacccgtt tagaggcccc aaggggttat gctagttatt 
     6001 gctcagcggt ggcagcagcc aactcagctt cctttcgggc tttgttagca gccggatctc 
     6061 agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt ttaacgatga ttccacacca 
     6121 ttttctgtgc atccagaata tgatgcagg 
//