LOCUS       pTCF\3        6152 bp    DNA   circular            19-FEB-2003
DEFINITION  Dd sol fragment(1-280) cut with NdeI & BamHI and ligated into similarly cut JA1.
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     CDS             complement(5306..2)
                     /vntifkey="4"
                     /label=Dd\Sol
     CDS             complement(4607..5062)
                     /vntifkey="4"
                     /label=f1\origin
     CDS             3699..4511
                     /vntifkey="4"
                     /label=Kan
     promoter        complement(74..90)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      2990..2990
                     /vntifkey="33"
                     /label=pBR322\origin
     CDS             477..1556
                     /vntifkey="4"
                     /label=lacI
     CDS             complement(5252..5305)
                     /vntifkey="4"
                     /label=BSP\41\sequence
BASE COUNT     1404 a      1664 c      1649 g      1435 t 
ORIGIN
        1 atatgtatat ctccttctta aagttaaaca aaattatttc tagaggggaa ttgttatccg 
       61 ctcacaattc ccctatagtg agtcgtatta atttcgcggg atcgagatct cgatcctcta 
      121 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat 
      181 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt 
      241 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca 
      301 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct 
      361 aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa 
      421 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga 
      481 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc 
      541 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga 
      601 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt 
      661 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg 
      721 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa 
      781 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc 
      841 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta 
      901 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct 
      961 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa 
     1021 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc 
     1081 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg 
     1141 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga 
     1201 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc 
     1261 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt 
     1321 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg 
     1381 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct 
     1441 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt 
     1501 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 
     1561 cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg accgatgccc 
     1621 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc 
     1681 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg 
     1741 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg 
     1801 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt 
     1861 ttcggcgaga agcaggccat tatcgccggc atggcggccc cacgggtgcg catgatcgtg 
     1921 ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa 
     1981 tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca 
     2041 acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg 
     2101 ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca 
     2161 cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc 
     2221 cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca 
     2281 tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac 
     2341 agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg 
     2401 gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg 
     2461 gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc 
     2521 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg 
     2581 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg 
     2641 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat 
     2701 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg 
     2761 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc 
     2821 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 
     2881 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 
     2941 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 
     3001 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 
     3061 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 
     3121 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 
     3181 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 
     3241 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 
     3301 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 
     3361 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 
     3421 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 
     3481 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 
     3541 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 
     3601 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgaacaataa 
     3661 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa 
     3721 cgtcttgctc taggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat 
     3781 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg 
     3841 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg 
     3901 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta 
     3961 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa acagcattcc 
     4021 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc 
     4081 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc 
     4141 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg 
     4201 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat 
     4261 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg 
     4321 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg 
     4381 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt 
     4441 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg 
     4501 atgagttttt ctaagaatta attcatgagc ggatacatat ttgaatgtat ttagaaaaat 
     4561 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaaat tgtaaacgtt 
     4621 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 
     4681 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 
     4741 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 
     4801 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 
     4861 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 
     4921 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 
     4981 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 
     5041 aatgcgccgc tacagggcgc gtcccattcg ccaatccgga tatagttcct cctttcagca 
     5101 aaaaacccct caagacccgt ttagaggccc caaggggtta tgctagttat tgctcagcgg 
     5161 tggcagcagc caactcagct tcctttcggg ctttgttagc agccggatct cagtggtggt 
     5221 ggtggtggtg ctcgagtgcg gccgcaagct tttaacgatg attccacacc attttctgtg 
     5281 catccagaat atgatgcagg gatccggatg aaggaggctc ctccttgccc catctcaggg 
     5341 tgaggggctc aggcagcccc tcatgttcca catggcatgt gtacttctgc tccttcccaa 
     5401 gaggcaccac cacagatgcc cacttctgga aggttccatc ccctgcaggc ctggtctcca 
     5461 caagctccat ttcctgggtc agctcctccc cattcaactg ccaggtcagg gtgatgtcag 
     5521 cagggtagaa gcccagggcc cagcacctca gggtgacatc accttcaggt ctgcggtgat 
     5581 gggtcacatg ggcctttggg ggatctgtgc gcagcagcgt agcattcccg ttcttcaggt 
     5641 atctgcggag ccactccacg cactcgccct ccaggtaggc ccggtctctc tctgcagcac 
     5701 cagcctgctc ccacttgcgt cgggtgatct gcgccgccat gtccgccgcc gtccacgttt 
     5761 tcaggtcttc gttcagggcg atgtaatcgc agccgtcgta ggcgaactgc cagtacccgc 
     5821 ggaggaggcg cccgtccgac tccacgtcac agccagccat ccactggagt gtgtgagagc 
     5881 cgcccgcgct ctggttgtag tagcgcagcg cggtcctcag gtccactcgg aaactctgct 
     5941 cattgccctt ggctctccgt gtctcccgct cccaatactc cggcccctcc tgctctatcc 
     6001 accgcgcccg cggctcatat ctcggattct ccgcgtcgct gtcgaagcgc acgaactccg 
     6061 tgttgtccac gtagccgact tccatgtacc ggggctcccc gaagccgggc cgggacacgg 
     6121 cggtgacgaa atatctaaga gaatgtgaac cc 
//