LOCUS       pTCF\30       6143 bp    DNA   circular            19-FEB-2003
DEFINITION  D(k) frag.  from JA 8 cut with NdeI/BamHI, ligated into similarly cut JA 60.
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     CDS             complement(3..845)
                     /vntifkey="4"
                     /label=Dk\Sol
     CDS             complement(5450..5905)
                     /vntifkey="4"
                     /label=f1\origin
     CDS             4542..5354
                     /vntifkey="4"
                     /label=Kan
     promoter        complement(917..933)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      3833..3833
                     /vntifkey="33"
                     /label=pBR322\origin
     CDS             1320..2399
                     /vntifkey="4"
                     /label=lacI
     CDS             complement(6056..6073)
                     /vntifkey="4"
                     /label=His\Tag
     CDS             complement(6095..6142)
                     /vntifkey="4"
                     /label=BSP\Sequence
BASE COUNT     1408 a      1648 c      1652 g      1435 t 
ORIGIN
        1 gatccggacg gaggaggctc ccatctcagg gtgaggggct caggcagccc ctcatggtac 
       61 acatggcatg tgtaattctg ctccttccca agaggcacca ccacagatgc ccacttctgg 
      121 aaggttccat cccctgcagg cctggtctcc acaagctcca tgtcctgggt cagctcctcc 
      181 ccattcaact gccaggtcag ggtgatgtca gcagggtaga agcccagggc ccagcacctc 
      241 agggtgactt caactttaga tctggggtga tgggtcacat gtgcctttgg ggaatctgtg 
      301 tgcagcagcg tcgcattccc gagctccagg tatctgcgga gccactccac gcacgtgccc 
      361 tccaggtagg cccggtctct ctctgcagca ccagcctgct cccacttgtg tttggtgatc 
      421 agcgccgcca tgtccgccgc cgtccacgtt ttcaggtctt cgttcagggc gatgtaatcg 
      481 cagccgtcgt atgcgaactg ctcgtacccg cggaggaggc gccagtccga ccccacgtca 
      541 cagccggaca gccgctggat cgtgtgagag ccaccctcgc tctggttgta gtagcggagc 
      601 agggtcctca ggtccactcg gaaactctgc tcattgccct tggcgatctg tgtctcccgt 
      661 tcccaatact ccggcccctc ctgctccatc caccgcaccc gcggctcatc tctcggattc 
      721 tccgcgtcgc tgtcgaagcg cacgaactcc gtgttgtcca cgtagccgac agagatgaac 
      781 cggggctctc cgaggccggg ccgggacacg acggtctcga aatatcttag tgaatgtggt 
      841 cccatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat 
      901 ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct 
      961 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta 
     1021 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg 
     1081 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt 
     1141 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt 
     1201 cctaatgcag gagtcgcata agggagagcg tcgagatccc ggacaccatc gaatggcgca 
     1261 aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg 
     1321 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt 
     1381 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg 
     1441 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt 
     1501 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg 
     1561 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac 
     1621 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg 
     1681 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca 
     1741 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt 
     1801 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc 
     1861 aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct 
     1921 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga 
     1981 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg 
     2041 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg 
     2101 ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat 
     2161 gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg 
     2221 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg 
     2281 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg 
     2341 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 
     2401 gagcgcaacg caattaatgt aagttagctc actcattagg caccgggatc tcgaccgatg 
     2461 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc 
     2521 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc 
     2581 tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt 
     2641 gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa 
     2701 cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccccacgggt gcgcatgatc 
     2761 gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat 
     2821 gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga 
     2881 gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca 
     2941 gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga 
     3001 acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc 
     3061 cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca 
     3121 tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg 
     3181 aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac 
     3241 atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac 
     3301 gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc 
     3361 agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 
     3421 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 
     3481 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg 
     3541 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatatgcg 
     3601 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc 
     3661 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 
     3721 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 
     3781 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 
     3841 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 
     3901 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 
     3961 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 
     4021 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 
     4081 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 
     4141 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 
     4201 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 
     4261 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 
     4321 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 
     4381 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 
     4441 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgaacaa 
     4501 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg 
     4561 aaacgtcttg ctctaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata 
     4621 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc 
     4681 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag 
     4741 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt 
     4801 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggg aaaacagcat 
     4861 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt 
     4921 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat 
     4981 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg 
     5041 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc 
     5101 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg 
     5161 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc 
     5221 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc 
     5281 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc 
     5341 tcgatgagtt tttctaagaa ttaattcatg agcggataca tatttgaatg tatttagaaa 
     5401 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga aattgtaaac 
     5461 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa 
     5521 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt 
     5581 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg 
     5641 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt 
     5701 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga 
     5761 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg 
     5821 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg 
     5881 cttaatgcgc cgctacaggg cgcgtcccat tcgccaatcc ggatatagtt cctcctttca 
     5941 gcaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctagt tattgctcag 
     6001 cggtggcagc agccaactca gcttcctttc gggctttgtt agcagccgga tctcagtggt 
     6061 ggtggtggtg gtgctcgagt gcggccgcaa gcttttaacg atgattccac accattttct 
     6121 gtgcatccag aatatgatgc agg 
//