LOCUS       pTCF\33-His   7348 bp    DNA   circular            17-JUN-2004
DEFINITION  TCF 33 with His tag fused to BSP.
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     insertion_seq   complement(12..2011)
                     /vntifkey="14"
                     /label=2\Kb\insert
     CDS             complement(1331..2011)
                     /vntifkey="4"
                     /label=DsRed1
     CDS             complement(6625..7080)
                     /vntifkey="4"
                     /label=f1\origin
     CDS             5717..6529
                     /vntifkey="4"
                     /label=Kan
     promoter        complement(2092..2108)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      5008..5008
                     /vntifkey="33"
                     /label=pBR322\origin
     CDS             2495..3574
                     /vntifkey="4"
                     /label=lacI
     CDS             complement(7231..7248)
                     /vntifkey="4"
                     /label=His\Tag
     misc_feature    complement(7303..7347)
                     /vntifkey="21"
                     /label=BSP
     misc_feature    complement(7273..7290)
                     /vntifkey="21"
                     /label=His\tag
     primer          7160..7178
                     /vntifkey="27"
                     /label=T7\rev
     primer          2088..2108
                     /vntifkey="27"
                     /label=T7\fwd
     primer          complement(100..120)
                     /vntifkey="27"
                     /label=MidSeq\primer
                     /note="TCF33 MidSeq 5p.ol"
BASE COUNT     1700 a      1896 c      1967 g      1785 t 
ORIGIN
        1 gatccggtac cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca 
       61 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc 
      121 tgtctcttga tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct 
      181 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc 
      241 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg 
      301 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg 
      361 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc 
      421 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagctggt 
      481 tctttccgcc tcaggactct tcctttttca atattattga agcatttatc agggttattg 
      541 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 
      601 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg 
      661 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 
      721 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 
      781 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 
      841 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 
      901 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 
      961 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 
     1021 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat 
     1081 ttacgcctta agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa 
     1141 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa 
     1201 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg 
     1261 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctaga 
     1321 gtcgcggccg ctacaggaac aggtggtggc ggccctcggt gcgctcgtac tgctccacga 
     1381 tggtgtagtc ctcgttgtgg gaggtgatgt ccagcttgga gtccacgtag tagtagccgg 
     1441 gcagctgcac gggcttcttg gccatgtaga tggacttgaa ctccaccagg tagtggccgc 
     1501 cgtccttcag cttcagggcc ttgtggatct cgcccttcag cacgccgtcg cgggggtaca 
     1561 ggcgctcggt ggaggcctcc cagcccatgg tcttcttctg cattacgggg ccgtcggagg 
     1621 ggaagttcac gccgatgaac ttcaccttgt agatgaagca gccgtcctgc agggaggagt 
     1681 cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc 
     1741 cctcggggaa ggacagcttc ttgtagtcgg ggatgtcggc ggggtgcttc acgtacacct 
     1801 tggagccgta ctggaactgg ggggacagga tgtcccaggc gaagggcagg gggccgccct 
     1861 tggtcacctt cagcttcacg gtgttgtggc cctcgtaggg gcggccctcg ccctcgccct 
     1921 cgatctcgaa ctcgtggccg ttcacggtgc cctccatgcg caccttgaag cgcatgaact 
     1981 ccttgatgac gttcttggag gagcgcacca tgctagccat atgtatatct ccttcttaaa 
     2041 gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag 
     2101 tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc 
     2161 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa 
     2221 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc 
     2281 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg 
     2341 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga 
     2401 gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata 
     2461 gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt 
     2521 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca 
     2581 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc 
     2641 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc 
     2701 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca 
     2761 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc 
     2821 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga 
     2881 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga 
     2941 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact 
     3001 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt 
     3061 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca 
     3121 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac 
     3181 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat 
     3241 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc 
     3301 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat 
     3361 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca 
     3421 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac 
     3481 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 
     3541 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt 
     3601 agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca 
     3661 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta 
     3721 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct 
     3781 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc 
     3841 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta 
     3901 tcgccggcat ggcggcccca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc 
     3961 taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt 
     4021 gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt 
     4081 tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga 
     4141 tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc 
     4201 gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt 
     4261 taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca 
     4321 tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg 
     4381 catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga 
     4441 cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg 
     4501 aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg 
     4561 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 
     4621 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg 
     4681 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc 
     4741 agagcagatt gtactgagag tgcaccatat atgcggtgtg aaataccgca cagatgcgta 
     4801 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 
     4861 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 
     4921 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 
     4981 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 
     5041 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 
     5101 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 
     5161 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 
     5221 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 
     5281 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 
     5341 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 
     5401 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 
     5461 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 
     5521 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 
     5581 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 
     5641 gaaaactcac gttaagggat tttggtcatg aacaataaaa ctgtctgctt acataaacag 
     5701 taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcta ggccgcgatt 
     5761 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca 
     5821 atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa 
     5881 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct 
     5941 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg 
     6001 gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga 
     6061 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc 
     6121 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg 
     6181 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt 
     6241 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac 
     6301 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat 
     6361 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg 
     6421 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa 
     6481 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aagaattaat 
     6541 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 
     6601 catttccccg aaaagtgcca cctgaaattg taaacgttaa tattttgtta aaattcgcgt 
     6661 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt 
     6721 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc 
     6781 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg 
     6841 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac 
     6901 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg 
     6961 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag 
     7021 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt 
     7081 cccattcgcc aatccggata tagttcctcc tttcagcaaa aaacccctca agacccgttt 
     7141 agaggcccca aggggttatg ctagttattg ctcagcggtg gcagcagcca actcagcttc 
     7201 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc 
     7261 cgcaagcttt cagtggtggt ggtggtggtg agaaccacca ccacgatgat tccacaccat 
     7321 tttctgtgca tccagaatat gatgcagg 
//