LOCUS       pTCF\33       7318 bp    DNA   circular             3-JUN-2004
DEFINITION  2 Kb fragment (containing DsRed1 gene) cut with NheI/KpnI, ligated into similarly cut JA 60.
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     insertion_seq   complement(5319..7318)
                     /vntifkey="14"
                     /label=2\Kb\insert
     CDS             complement(6638..7318)
                     /vntifkey="4"
                     /label=DsRed1
     CDS             complement(4614..5069)
                     /vntifkey="4"
                     /label=f1\origin
     CDS             3706..4518
                     /vntifkey="4"
                     /label=Kan
     promoter        complement(81..97)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      2997..2997
                     /vntifkey="33"
                     /label=pBR322\origin
     CDS             484..1563
                     /vntifkey="4"
                     /label=lacI
     CDS             complement(5220..5237)
                     /vntifkey="4"
                     /label=His\Tag
     CDS             complement(5259..5306)
                     /vntifkey="4"
                     /label=BSP\Sequence
     primer          5149..5167
                     /vntifkey="27"
                     /label=T7\Rev
     primer          77..97
                     /vntifkey="27"
                     /label=T7\fwd
BASE COUNT     1695 a      1889 c      1954 g      1780 t 
ORIGIN
        1 gctagccata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg 
       61 ttatccgctc acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatctcga 
      121 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg 
      181 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg 
      241 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct 
      301 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct 
      361 gcttcctaat gcaggagtcg cataagggag agcgtcgaga tcccggacac catcgaatgg 
      421 cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg 
      481 aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc 
      541 gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa 
      601 gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa 
      661 cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt 
      721 gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta 
      781 gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc 
      841 agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc 
      901 tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt 
      961 attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac 
     1021 cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct 
     1081 ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac 
     1141 tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc 
     1201 actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag 
     1261 tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc 
     1321 tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc 
     1381 agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg 
     1441 cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc 
     1501 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 
     1561 cagtgagcgc aacgcaatta atgtaagtta gctcactcat taggcaccgg gatctcgacc 
     1621 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat 
     1681 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc 
     1741 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc 
     1801 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac 
     1861 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccccac gggtgcgcat 
     1921 gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca 
     1981 gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac 
     2041 ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa 
     2101 gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg 
     2161 tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg 
     2221 gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg 
     2281 ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc 
     2341 catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct 
     2401 taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct 
     2461 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta 
     2521 ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 
     2581 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 
     2641 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg 
     2701 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatata 
     2761 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg 
     2821 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 
     2881 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 
     2941 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 
     3001 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 
     3061 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 
     3121 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 
     3181 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 
     3241 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 
     3301 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 
     3361 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 
     3421 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 
     3481 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 
     3541 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 
     3601 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 
     3661 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa 
     3721 cgggaaacgt cttgctctag gccgcgatta aattccaaca tggatgctga tttatatggg 
     3781 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg 
     3841 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt 
     3901 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag 
     3961 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca 
     4021 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca 
     4081 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc 
     4141 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat 
     4201 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt 
     4261 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt 
     4321 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga 
     4381 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa 
     4441 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg 
     4501 atgctcgatg agtttttcta agaattaatt catgagcgga tacatatttg aatgtattta 
     4561 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaaattgt 
     4621 aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa 
     4681 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt 
     4741 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa 
     4801 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag 
     4861 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt 
     4921 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg 
     4981 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc 
     5041 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca atccggatat agttcctcct 
     5101 ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc tagttattgc 
     5161 tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc cggatctcag 
     5221 tggtggtggt ggtggtgctc gagtgcggcc gcaagctttt aacgatgatt ccacaccatt 
     5281 ttctgtgcat ccagaatatg atgcagggat ccggtaccag ccgattgtct gttgtgccca 
     5341 gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg 
     5401 ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc 
     5461 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct 
     5521 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag 
     5581 ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca 
     5641 tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact 
     5701 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc 
     5761 ctaactgaca cacattccac agctggttct ttccgcctca ggactcttcc tttttcaata 
     5821 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 
     5881 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc 
     5941 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 
     6001 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 
     6061 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 
     6121 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 
     6181 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 
     6241 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 
     6301 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 
     6361 ttttaacaaa atattaacgc ttacaattta cgccttaaga tacattgatg agtttggaca 
     6421 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc 
     6481 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt 
     6541 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa 
     6601 atgtggtatg gctgattatg atctagagtc gcggccgcta caggaacagg tggtggcggc 
     6661 cctcggtgcg ctcgtactgc tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca 
     6721 gcttggagtc cacgtagtag tagccgggca gctgcacggg cttcttggcc atgtagatgg 
     6781 acttgaactc caccaggtag tggccgccgt ccttcagctt cagggccttg tggatctcgc 
     6841 ccttcagcac gccgtcgcgg gggtacaggc gctcggtgga ggcctcccag cccatggtct 
     6901 tcttctgcat tacggggccg tcggagggga agttcacgcc gatgaacttc accttgtaga 
     6961 tgaagcagcc gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga 
     7021 agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcttg tagtcgggga 
     7081 tgtcggcggg gtgcttcacg tacaccttgg agccgtactg gaactggggg gacaggatgt 
     7141 cccaggcgaa gggcaggggg ccgcccttgg tcaccttcag cttcacggtg ttgtggccct 
     7201 cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggtgccct 
     7261 ccatgcgcac cttgaagcgc atgaactcct tgatgacgtt cttggaggag cgcaccat 
//