LOCUS       TCF\9        6152 bp    DNA   circular            19-FEB-2003
DEFINITION  HLA A0205  cut with NheI/BamHI, ligated into similarly cut  JA 60.
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     CDS             complement(4613..5068)
                     /vntifkey="4"
                     /label=f1\origin
     CDS             3705..4517
                     /vntifkey="4"
                     /label=Kan
     promoter        complement(80..96)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      2996..2996
                     /vntifkey="33"
                     /label=pBR322\origin
     CDS             483..1562
                     /vntifkey="4"
                     /label=lacI
     terminator      5105..5147
                     /vntifkey="43"
                     /label=T7\terminator
     CDS             complement(5312..6151)
                     /vntifkey="4"
                     /label=A*0205\Sol
     CDS             complement(5258..5311)
                     /vntifkey="4"
                     /label=BSP\sequence
BASE COUNT     1394 a      1657 c      1670 g      1431 t 
ORIGIN
        1 ctagccatat gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt 
       61 tatccgctca caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat 
      121 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc 
      181 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc 
      241 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc 
      301 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg 
      361 cttcctaatg caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc 
      421 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga 
      481 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg 
      541 tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag 
      601 cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac 
      661 agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg 
      721 tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag 
      781 aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca 
      841 gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct 
      901 gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta 
      961 ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc 
     1021 agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg 
     1081 gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact 
     1141 ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca 
     1201 ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt 
     1261 ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct 
     1321 catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca 
     1381 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc 
     1441 ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc 
     1501 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 
     1561 agtgagcgca acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg 
     1621 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc 
     1681 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg 
     1741 ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg 
     1801 cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc 
     1861 aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccccacg ggtgcgcatg 
     1921 atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag 
     1981 aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc 
     2041 tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag 
     2101 tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt 
     2161 ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg 
     2221 tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt 
     2281 tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc 
     2341 atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt 
     2401 aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg 
     2461 gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac 
     2521 cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg 
     2581 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 
     2641 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga 
     2701 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatat 
     2761 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc 
     2821 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 
     2881 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 
     2941 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 
     3001 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 
     3061 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 
     3121 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 
     3181 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 
     3241 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 
     3301 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 
     3361 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 
     3421 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 
     3481 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 
     3541 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 
     3601 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgaa 
     3661 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac 
     3721 gggaaacgtc ttgctctagg ccgcgattaa attccaacat ggatgctgat ttatatgggt 
     3781 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga 
     3841 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta 
     3901 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc 
     3961 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag 
     4021 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag 
     4081 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg 
     4141 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt 
     4201 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt 
     4261 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt 
     4321 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat 
     4381 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac 
     4441 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga 
     4501 tgctcgatga gtttttctaa gaattaattc atgagcggat acatatttga atgtatttag 
     4561 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaaattgta 
     4621 aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac 
     4681 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg 
     4741 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa 
     4801 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt 
     4861 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt 
     4921 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga 
     4981 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc 
     5041 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccaa tccggatata gttcctcctt 
     5101 tcagcaaaaa acccctcaag acccgtttag aggccccaag gggttatgct agttattgct 
     5161 cagcggtggc agcagccaac tcagcttcct ttcgggcttt gttagcagcc ggatctcagt 
     5221 ggtggtggtg gtggtgctcg agtgcggccg caagctttta acgatgattc cacaccattt 
     5281 tctgtgcatc cagaatatga tgcagggatc cgggctggga agacggctcc catctcaggg 
     5341 tgaggggctt gggcaaaccc tcatgctgca catggcaggt gtatctctgc tcctgtccag 
     5401 aaggcaccac cacagccgcc cacttctgga aggttccatc ccctgcaggc ctggtctcca 
     5461 cgagctccgt gtcctgggtc tggtcctccc catcccgctg ccaggtcagt gtgatctccg 
     5521 cagggtagaa gctcagggcc cagcacctca gggtggcttc atggtcagag acagcgtggt 
     5581 gagtcatatg cgttttgggg gcgtccgtgc gctgcagcgt ctccttcccg ttctccaggt 
     5641 atctgcggag ccactccacg cacgtgccct ccaggtaggc tctccactgc tccgccacat 
     5701 gggccgcctc ccacttgtgc ttggtggtct gagctgccat gtccgccgcg gtccaagagc 
     5761 gcaggtcctc tttcagggcg atgtaatcct tgccgtcgta ggcgtactgg tggtacccgc 
     5821 gcaggaagcg ccagtccgac cccacgtcgc agccatacat cctctggagg gtgtgagaac 
     5881 cggcctcgct ctggttgtag tagccgcgca gggtccccag gtccactcgg tgagtctgtg 
     5941 agtgggcctt cactttccgt gtctccccgt cccaatactc cggaccctcc tgctctatcc 
     6001 acggcgcccg cggctccatc ctccggctcg cggcgtcgct gtcgaaccgc acgaactgcg 
     6061 tgtcgtccac gtagcccact gcgatgaagc ggggctcccc gcggccgggc cgggacacgg 
     6121 aggtgtagaa atatctcata gaatgagaac cg 
//