LOCUS TCF\20 6178 bp DNA circular 19-FEB-2003
DEFINITION Kd-mut cut with NdeI/BamHI, ligated into similarly cut JA 33.
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT VNTDBDATE|294771279|
COMMENT VNTNAME|TCF 20|
COMMENT VNTREPLTYPE|Plasmid
COMMENT VNTEXTCHREPL|Bacteria
COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES Location/Qualifiers
CDS 3733..4545
/vntifkey="4"
/label=Kan
rep_origin 3024..3024
/vntifkey="33"
/label=pBR322\origin
CDS 511..1590
/vntifkey="4"
/label=lacI
CDS complement(5..37)
/vntifkey="4"
/label=T7\Tag
promoter complement(108..124)
/vntifkey="30"
/label=T7\Promoter
CDS 81..106
/vntifkey="4"
/label=Lac\Operator
CDS complement(5340..6176)
/vntifkey="4"
/label=Kd\Sol
CDS 5286..5339
/vntifkey="4"
/label=BSP41\sequence
BASE COUNT 1422 a 1655 c 1659 g 1442 t
ORIGIN
1 tatgacccat ttgctgtcca ccagtcatgc tagccatggt atatctcctt cttaaagtta
61 aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt
121 attaatttcg cgggatcgag atctcgatcc tctacgccgg acgcatcgtg gccggcatca
181 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
241 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
301 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc
361 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
421 gtcgagatcc cggacaccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc
481 ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca
541 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt
601 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
661 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
721 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
781 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
841 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac
901 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc
961 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc
1021 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
1081 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
1141 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
1201 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
1261 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
1321 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa
1381 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
1441 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg
1501 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
1561 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct
1621 cactcattag gcaccgggat ctcgaccgat gcccttgaga gccttcaacc cagtcagctc
1681 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
1741 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg
1801 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc
1861 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc
1921 cggcatggcg gccccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg
1981 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag
2041 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg
2101 tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg
2161 catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg
2221 gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc
2281 ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct
2341 ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc
2401 agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt
2461 aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc
2521 gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg
2581 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
2641 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
2701 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
2761 cagattgtac tgagagtgca ccatatatgc ggtgtgaaat accgcacaga tgcgtaagga
2821 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
2881 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
2941 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
3001 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
3061 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
3121 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
3181 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
3241 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
3301 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
3361 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
3421 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
3481 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
3541 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
3601 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
3661 actcacgtta agggattttg gtcatgaaca ataaaactgt ctgcttacat aaacagtaat
3721 acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctctaggcc gcgattaaat
3781 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
3841 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
3901 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
3961 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
4021 ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca
4081 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
4141 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
4201 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
4261 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
4321 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
4381 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
4441 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
4501 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaaga attaattcat
4561 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
4621 tccccgaaaa gtgccacctg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa
4681 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
4741 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
4801 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
4861 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
4921 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
4981 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
5041 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
5101 ttcgccaatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
5161 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
5221 cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca
5281 agcttttaac gatgattcca caccattttc tgtgcatcca gaatatgatg cagggatccg
5341 gatggaggaa gttcccatct cagggtgaga ggctcaggca gccccttatg gtgcacatgg
5401 catgtgtaat tctgctcctt cccaagaggc accaccacag ctgcccactt ctggaaggtt
5461 ccatcccctg caggcctggt ctctacaagc tccatgtcct gggtcaggtc ctccccattc
5521 aactgccagg tcagggtgat atcagcaggg tagaagccca gggcccagca cctcagggtg
5581 acatcaactt gagatctggg gtgataggtc acatgggcct ttggggaatc tgtgcgcagc
5641 agcgtctcat tcccgagctc caggtatctg cggagccact ccacgcactc gccctctagg
5701 taggccctgt aatactctgc atcaccagcc tgctcccact tgcgtctggt gatcagcgcc
5761 gccgtgtccg ccgccgtcca cgttttcagg tcttcgttca gggcgatgta atcgcggccg
5821 tcgtaggcga actgatggta cccgcggagg aggcgccagt ccgaccccac gtcacagccg
5881 aacatccgct ggaacgtgtg agagccgccc ttgctctggt tgtagtatct ctgtgcggtc
5941 ctcaggctca ctcggaacca ctgctcatcg ctcttggctc tctgtgtctg ctcctcccaa
6001 tactccggcc cctcctgctc catccacggc gcccgcggct caaatctcgg attatccgcg
6061 tcgctgtcga agcgcacgaa ctgcgtgtcg tccacgtagc cgacagcgat gaaccggggc
6121 tccccgaggc cgggccggga cacggcggtg acgaaatatc taagagaatg tggaccca
//