LOCUS TCF\23 6149 bp DNA circular 19-FEB-2003 DEFINITION H-2Dq cut with NheI/BamHI, ligated into similarly cut JA 60. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDBDATE|294771281| COMMENT VNTNAME|TCF 23| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "TCF 23" 0 6149 COMMENT (CDBMol 0 0 1 843 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList COMMENT #0=(CFragment 3 1 1 177831312 0 "" 0 "" "" "" "" "" "" "H-2Dq Sol" "" 0 COMMENT 0 1 843 1 843 9 851 0 "" "" "" "" "" "" (CObList) COMMENT (CTerm 1 0 0 "NheI" "" 1 0 0 0 0 0 0 0 0 0) COMMENT (CTerm 1 1 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) COMMENT #1=(CFragment 3 1 0 0 0 "" 0 "" "" "" "" "" "" "JA 60" "" 0 0 1 5306 COMMENT 844 6149 283 5588 0 "" "" "" "" "" "" (CObList) COMMENT (CTerm 1 0 0 "NheI" "" 1 0 0 0 0 0 0 0 0 0) COMMENT (CTerm 1 1 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 1 0)) (CObList)) COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 1 0 3 33 40 50 0 1 0 COMMENT (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" COMMENT "PstI" "SmaI" "XmaI" "BglII" "DraIII" "EcoRV" "KpnI" "NdeI" "NheI" COMMENT "SacI" "XbaI" "XhoI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 0 0 0 0 0) (CMolPar 0 0 0 0 0 1 6149 0 0 0 0 0 0 0 0) COMMENT (CStringList) (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList COMMENT #2=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 1657 0 3592 0 4092 0) COMMENT #3=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 3 790 0 4852 0 6081 0) COMMENT #4=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1 0) COMMENT #5=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 4671 0) COMMENT #6=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0) COMMENT #7=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 6096 0) COMMENT #8=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 0) COMMENT #9=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 139 0 388 0) COMMENT #10=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 4854 0) COMMENT #11=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 4852 0) COMMENT #12=(CRSite (CStringList) "BglII" "agatct" 2 0 2 259 0 955 0) COMMENT #13=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 1 5681 0) COMMENT #14=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 2127 0) COMMENT #15=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0) COMMENT #16=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 851 0) COMMENT #17=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 844 0) COMMENT #18=(CRSite (CStringList) "SacI" "gagctc" 6 0 0) COMMENT #19=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 889 0) COMMENT #20=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 6081 0)) COMMENT (CObList COMMENT #21=(CFSignal (CObList) "H-2Dq" 4 0 1 6 842 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #22=(CFSignal (CObList) "f1 origin" 4 0 1 5456 5911 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #23=(CFSignal (CObList) "Kan" 4 0 0 4548 5360 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #24=(CFSignal (CObList) "T7 promoter" 30 0 1 923 939 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #25=(CFSignal (CObList) "pBR322 origin" 33 0 0 3839 3839 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #26=(CFSignal (CObList) "lacI" 4 0 0 1326 2405 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #27=(CFSignal (CObList) "His Tag" 4 0 1 6062 6079 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #28=(CFSignal (CObList) "BSP Sequence" 4 0 1 6101 6148 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) COMMENT (CObList) (CObList) COMMENT (CTextView 0 COMMENT #29=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #30=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "TCF 23" 2) 5 "" 0 COMMENT 4) COMMENT #31=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA Plasmid 'TCF 23'" 1) COMMENT #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "H-2Dq cut with NheI/BamHI, ligated into similarly cut JA 60" COMMENT 1) COMMENT #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Foreign object. Author: UNKNOWN. Original author: UNKNOWN" COMMENT 1) COMMENT #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Creation Date: ? 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COMMENT #55=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "molecule position: from 844 to 6149" 1) COMMENT #56=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #57=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " NheI site #1" 1))) "Left Terminus") COMMENT #58=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #59=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " BamHI site #1" 1))) "Right Terminus"))) COMMENT "#2: FRAGMENT of JA 60 "))) COMMENT "Component Fragments") COMMENT #60=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #61=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #62=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 6 (3 #21# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #63=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 6 End: 842 (Complementary)" 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COMMENT 3.48466 0 -10 5.23214 1.07863 #256#) COMMENT #258=(CScratch COMMENT (CWidget 3 (1 #3# 3) 1 2 0 0 #144# 0 100) COMMENT (LOGPEN 0 8 10053171) 8 1 0.0703935 1.9 COMMENT 0.082322 1) COMMENT #259=(CLabel (CWidget 0 (0 0) 1 2 0 0 #136# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 59 22 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Ava}I (6081)" "@N (@S)" 5 -4.4746 COMMENT 15.681 0 -10 5.18385 1.07863 #258#))))) COMMENT #260=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList)) COMMENT #261=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList))))) COMMENT (CSeqView 3 33 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers CDS complement(6..842) /vntifkey="4" /label=H-2Dq CDS complement(5456..5911) /vntifkey="4" /label=f1\origin CDS 4548..5360 /vntifkey="4" /label=Kan promoter complement(923..939) /vntifkey="30" /label=T7\promoter rep_origin 3839..3839 /vntifkey="33" /label=pBR322\origin CDS 1326..2405 /vntifkey="4" /label=lacI CDS complement(6062..6079) /vntifkey="4" /label=His\Tag CDS complement(6101..6148) /vntifkey="4" /label=BSP\Sequence BASE COUNT 1408 a 1654 c 1655 g 1432 t ORIGIN 1 gatccggacg gaggaggctc ccatctcagg gtgaggggct caggcagccc ctcatggtac 61 acacggcatg tgtaattctg ctccttccca agaggcacca ccacagatgc ccacttctgg 121 aaggttccat cccctgcagg cctggtctcc acaagctcca tgtcctgggt cagctcctcc 181 ccattcaact gccaggtcag ggtgatgtca gcagggtaga agcccagggc ccagcacctc 241 agggtgactt cacctttaga tctggggtga tgggtcacat gtgcctttgg ggaatctgtg 301 cgcagcagcg tcgcgttccc gttcttcagg tatctgtgga gccactccac gcactcgccc 361 tccaggtagg ccttgtaatg ctctgcagca ccaccctgct cccacttgcg tcgggtgatc 421 tgcgccgcca tgtccgccgc cgtccacgtt ttcaggtctt cgttcagggc gatgtaatcg 481 aggccgtcgt aggcgaactg ctcgtacccg cggaggaggc gcccgtccaa ccccacgtca 541 cagccgtaca tccgctggat cgtgtgagag ccgcccgcgc tctggttgta gtagcgcagc 601 gcggtcctca ggctcactcg gaaccactgc tcattgtcct tggcgatctg cgtgatccgc 661 tcccaatact ccggcccctc ctgctccatc cacggcgccc gcggctcata tctcggattc 721 tccgcgtcgc tgtcgaagcg cacgaactcc ttgttgtcca catagccgac agagatgtac 781 cggggctccc cgaggccggg ccgggacacg gcggtctcga aataacgcat agaatgtgga 841 ccgctagcca tatgtatatc tccttcttaa agttaaacaa aattatttct agaggggaat 901 tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatctc 961 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg 1021 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag 1081 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat 1141 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg 1201 ctgcttccta atgcaggagt cgcataaggg agagcgtcga gatcccggac accatcgaat 1261 ggcgcaaaac ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg 1321 tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga 1381 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg 1441 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca 1501 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa 1561 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg 1621 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg 1681 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg 1741 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta 1801 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc 1861 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg 1921 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg 1981 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc 2041 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg 2101 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca 2161 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa 2221 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt 2281 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc 2341 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 2401 ggcagtgagc gcaacgcaat taatgtaagt tagctcactc attaggcacc gggatctcga 2461 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact 2521 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca 2581 gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg 2641 tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc 2701 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggcccc acgggtgcgc 2761 atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag 2821 cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg 2881 acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg 2941 aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc 3001 tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc 3061 tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca 3121 tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc 3181 cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga aaaaaccgcc 3241 cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag 3301 ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt 3361 taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc 3421 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc 3481 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc 3541 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata 3601 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc 3661 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 3721 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 3781 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 3841 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 3901 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 3961 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 4021 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 4081 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 4141 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 4201 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 4261 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 4321 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 4381 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 4441 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 4501 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc 4561 aacgggaaac gtcttgctct aggccgcgat taaattccaa catggatgct gatttatatg 4621 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg 4681 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg 4741 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca 4801 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccgggaaaa 4861 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg 4921 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc 4981 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg 5041 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac 5101 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta 5161 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc 5221 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga 5281 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt 5341 tgatgctcga tgagtttttc taagaattaa ttcatgagcg gatacatatt tgaatgtatt 5401 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaaatt 5461 gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt 5521 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg 5581 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc 5641 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca 5701 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga 5761 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa 5821 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc 5881 gccgcgctta atgcgccgct acagggcgcg tcccattcgc caatccggat atagttcctc 5941 ctttcagcaa aaaacccctc aagacccgtt tagaggcccc aaggggttat gctagttatt 6001 gctcagcggt ggcagcagcc aactcagctt cctttcgggc tttgttagca gccggatctc 6061 agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt ttaacgatga ttccacacca 6121 ttttctgtgc atccagaata tgatgcagg //