LOCUS TCF\30 6143 bp DNA circular 19-FEB-2003 DEFINITION D(k) frag. from JA 8 cut with NdeI/BamHI, ligated into similarly cut JA 60. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDBDATE|294771282| COMMENT VNTNAME|TCF 30| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "TCF 30" 0 6143 COMMENT (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList COMMENT #0=(CFragment 3 1 0 170 0 "" 0 "" "" "" "" "" "" "JA 8" "" 0 0 1 844 1 COMMENT 844 3657 1 0 "" "" "" "" "" "" (CObList) COMMENT (CTerm 1 0 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) COMMENT (CTerm 1 1 0 "NdeI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) COMMENT #1=(CFragment 3 1 0 170 0 "" 0 "" "" "" "" "" "" "JA 60" "" 0 0 1 5299 COMMENT 845 6143 290 5588 0 "" "" "" "" "" "" (CObList) COMMENT (CTerm 1 0 0 "NdeI" "" 1 0 0 0 0 0 0 0 0 0) COMMENT (CTerm 1 1 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 1 0)) (CObList)) COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 1 0 3 33 40 50 0 1 0 COMMENT (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" COMMENT "PstI" "SmaI" "XmaI" "BglII" "DraIII" "EcoRV" "KpnI" "NdeI" "NheI" COMMENT "SacI" "XbaI" "XhoI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 0 0 0 0 0) (CMolPar 0 0 0 0 0 1 6143 0 0 0 0 0 0 0 0) COMMENT (CStringList) (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList COMMENT #2=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 1651 0 3586 0 4086 0) COMMENT #3=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 3 319 0 4846 0 6075 0) COMMENT #4=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1 0) COMMENT #5=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 4665 0) COMMENT #6=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0) COMMENT #7=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 6090 0) COMMENT #8=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 0) COMMENT #9=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 139 0 388 0) COMMENT #10=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 4848 0) COMMENT #11=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 4846 0) COMMENT #12=(CRSite (CStringList) "BglII" "agatct" 2 0 2 259 0 949 0) COMMENT #13=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 1 5675 0) COMMENT #14=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 2121 0) COMMENT #15=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0) COMMENT #16=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 845 0) COMMENT #17=(CRSite (CStringList) "NheI" "gctagc" 2 0 0) COMMENT #18=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 326 0) COMMENT #19=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 883 0) COMMENT #20=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 6075 0)) COMMENT (CObList COMMENT #21=(CFSignal (CObList) "Dk Sol" 4 0 1 3 845 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #22=(CFSignal (CObList) "f1 origin" 4 0 1 5450 5905 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #23=(CFSignal (CObList) "Kan" 4 0 0 4542 5354 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #24=(CFSignal (CObList) "T7 promoter" 30 0 1 917 933 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #25=(CFSignal (CObList) "pBR322 origin" 33 0 0 3833 3833 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #26=(CFSignal (CObList) "lacI" 4 0 0 1320 2399 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #27=(CFSignal (CObList) "His Tag" 4 0 1 6056 6073 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #28=(CFSignal (CObList) "BSP Sequence" 4 0 1 6095 6142 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) COMMENT (CObList) (CObList) COMMENT (CTextView 0 COMMENT #29=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #30=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "TCF 30" 2) 5 "" 0 COMMENT 4) COMMENT #31=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA Plasmid 'TCF 30'" 1) COMMENT #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "D(k) frag. from JA 8 cut with NdeI/BamHI, ligated into similarly cut JA 60" COMMENT 1) COMMENT #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Foreign object. Author: UNKNOWN. Original author: UNKNOWN" COMMENT 1) COMMENT #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Creation Date: ? (no data)" 1) COMMENT #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Last Modified: 02/19/03 04:54 PM" 1) COMMENT #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "length: 6143 bp" 1) COMMENT #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Constructed" 1) COMMENT #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "form: Circular" 1))) "General Description") COMMENT #40=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList)) COMMENT "Standard Fields") COMMENT #41=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" COMMENT 0 0) COMMENT #42=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) COMMENT "Annotations") COMMENT #43=(CFolderPar COMMENT (CGroupPar (CParagraph 10 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #44=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (5 #0# 0) 1 2 1 0 195) COMMENT (CObjectList COMMENT #45=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "parent position: from 3657 to 1" 1) COMMENT #46=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "original length: 844" 1) COMMENT #47=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "molecule position: from 1 to 844" 1) COMMENT #48=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #49=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " BamHI site #1" 1))) "Left Terminus") COMMENT #50=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #51=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " NdeI site #1" 1))) "Right Terminus"))) COMMENT "#1: FRAGMENT of JA 8 ") COMMENT #52=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (5 #1# 0) 1 2 1 0 195) COMMENT (CObjectList COMMENT #53=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "parent position: from 290 to 5588" 1) COMMENT #54=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "original length: 5299" 1) COMMENT #55=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "molecule position: from 845 to 6143" 1) COMMENT #56=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #57=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " NdeI site #1" 1))) "Left Terminus") COMMENT #58=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #59=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " BamHI site #1" 1))) "Right Terminus"))) COMMENT "#2: FRAGMENT of JA 60 "))) COMMENT "Component Fragments") COMMENT #60=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #61=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #62=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3 (3 #21# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #63=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3 End: 845 (Complementary)" COMMENT 1))) "Dk Sol") COMMENT #64=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1320 (3 #26# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #65=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1320 End: 2399" 1))) "lacI") COMMENT #66=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4542 (3 #23# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #67=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4542 End: 5354" 1))) "Kan") COMMENT #68=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5450 (3 #22# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #69=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5450 End: 5905 (Complementary)" COMMENT 1))) "f1 origin") COMMENT #70=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 6056 (3 #27# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #71=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 6056 End: 6073 (Complementary)" COMMENT 1))) "His Tag") COMMENT #72=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 6095 (3 #28# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #73=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 6095 End: 6142 (Complementary)" COMMENT 1))) "BSP Sequence"))) "CDS (6 total)") COMMENT #74=(CFolderPar COMMENT (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #75=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 917 (3 #24# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #76=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 917 End: 933 (Complementary)" COMMENT 1))) "T7 promoter"))) COMMENT "Promoter Prokaryotic (1 total)") COMMENT #77=(CFolderPar COMMENT (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #78=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3833 (3 #25# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #79=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3833 End: 3833" 1))) COMMENT "pBR322 origin"))) COMMENT "Replication Origin (1 total)"))) "Feature Map") COMMENT #80=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #81=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 53982616 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #82=(CGroupPar COMMENT (CParagraph 0 (10 #2# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #83=(CLinePar COMMENT (CParagraph 0 (1 #2# 1) 1 2 2 0 191) COMMENT " N1: 1651 " 1) COMMENT #84=(CLinePar COMMENT (CParagraph 0 (1 #2# 2) 1 2 2 0 191) COMMENT " N2: 3586 " 1) COMMENT #85=(CLinePar COMMENT (CParagraph 0 (1 #2# 3) 1 2 2 0 191) COMMENT " N3: 4086 " 1))))) "ApaLI: 3 sites") 1 COMMENT 5 "GTGCAC" "CACGTG") COMMENT #86=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 53451448 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #87=(CGroupPar COMMENT (CParagraph 0 (10 #3# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #88=(CLinePar COMMENT (CParagraph 0 (1 #3# 1) 1 2 2 0 191) COMMENT " N1: 319 " 1) COMMENT #89=(CLinePar COMMENT (CParagraph 0 (1 #3# 2) 1 2 2 0 191) COMMENT " N2: 4846 " 1) COMMENT #90=(CLinePar COMMENT (CParagraph 0 (1 #3# 3) 1 2 2 0 191) COMMENT " N3: 6075 " 1))))) "AvaI: 3 sites") 1 5 COMMENT "CYCGRG" "GRGCYC") COMMENT #91=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 53888392 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #92=(CGroupPar COMMENT (CParagraph 0 (10 #4# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #93=(CLinePar COMMENT (CParagraph 0 (1 #4# 1) 1 2 2 0 191) COMMENT " N1: 1 " 1))))) "BamHI: 1 site") 1 5 COMMENT "GGATCC" "CCTAGG") COMMENT #94=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 53967640 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #95=(CGroupPar COMMENT (CParagraph 0 (10 #12# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #96=(CLinePar COMMENT (CParagraph 0 (1 #12# 1) 1 2 2 0 191) COMMENT " N1: 259 " 1) COMMENT #97=(CLinePar COMMENT (CParagraph 0 (1 #12# 2) 1 2 2 0 191) COMMENT " N2: 949 " 1))))) "BglII: 2 sites") 1 5 COMMENT "AGATCT" "TCTAGA") COMMENT #98=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 53707928 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #99=(CGroupPar COMMENT (CParagraph 0 (10 #5# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #100=(CLinePar COMMENT (CParagraph 0 (1 #5# 1) 1 2 2 0 191) COMMENT " N1: 4665 " 1))))) "ClaI: 1 site") 2 4 COMMENT "ATCGAT" "TAGCTA") COMMENT #101=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 54041016 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #102=(CGroupPar COMMENT (CParagraph 0 (10 #13# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #103=(CLinePar COMMENT (CParagraph 0 (1 #13# 1) 1 2 2 0 191) COMMENT " N1: 5675 " 1))))) "DraIII: 1 site") COMMENT 6 3 "CACNNNGTG" "GTGNNNCAC") COMMENT #104=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 53674136 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "EcoRI: 0 sites") 1 5 "GAATTC" COMMENT "CTTAAG") COMMENT #105=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 53490712 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #106=(CGroupPar COMMENT (CParagraph 0 (10 #14# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #107=(CLinePar COMMENT (CParagraph 0 (1 #14# 1) 1 2 2 0 191) COMMENT " N1: 2121 " 1))))) "EcoRV: 1 site") COMMENT 3 3 "GATATC" "CTATAG") COMMENT #108=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 53733048 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #109=(CGroupPar COMMENT (CParagraph 0 (10 #7# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #110=(CLinePar COMMENT (CParagraph 0 (1 #7# 1) 1 2 2 0 191) COMMENT " N1: 6090 " 1))))) "HindIII: 1 site") COMMENT 1 5 "AAGCTT" "TTCGAA") COMMENT #111=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 53717432 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "KpnI: 0 sites") 5 1 "GGTACC" COMMENT "CCATGG") COMMENT #112=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 50895048 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "NcoI: 0 sites") 1 5 "CCATGG" COMMENT "GGTACC") COMMENT #113=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 50906952 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #114=(CGroupPar COMMENT (CParagraph 0 (10 #16# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #115=(CLinePar COMMENT (CParagraph 0 (1 #16# 1) 1 2 2 0 191) COMMENT " N1: 845 " 1))))) "NdeI: 1 site") 2 COMMENT 4 "CATATG" "GTATAC") COMMENT #116=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 53758376 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "NheI: 0 sites") 1 5 "GCTAGC" COMMENT "CGATCG") COMMENT #117=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 53756536 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #118=(CGroupPar COMMENT (CParagraph 0 (10 #9# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #119=(CLinePar COMMENT (CParagraph 0 (1 #9# 1) 1 2 2 0 191) COMMENT " N1: 139 " 1) COMMENT #120=(CLinePar COMMENT (CParagraph 0 (1 #9# 2) 1 2 2 0 191) COMMENT " N2: 388 " 1))))) "PstI: 2 sites") 5 COMMENT 1 "CTGCAG" 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(1 #19# 1) 1 2 2 0 191) COMMENT " N1: 883 " 1))))) "XbaI: 1 site") 1 COMMENT 5 "TCTAGA" "AGATCT") COMMENT #130=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 53901096 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #131=(CGroupPar COMMENT (CParagraph 0 (10 #20# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #132=(CLinePar COMMENT (CParagraph 0 (1 #20# 1) 1 2 2 0 191) COMMENT " N1: 6075 " 1))))) "XhoI: 1 site") 1 COMMENT 5 "CTCGAG" "GAGCTC") COMMENT #133=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 53938040 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #134=(CGroupPar COMMENT (CParagraph 0 (10 #11# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #135=(CLinePar COMMENT (CParagraph 0 (1 #11# 1) 1 2 2 0 191) COMMENT " N1: 4846 " 1))))) "XmaI: 1 site") 1 COMMENT 5 "CCCGGG" "GGGCCC"))) "Restriction Map")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #136=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 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COMMENT 3.48466 0 -10 5.26434 1.07863 #256#) COMMENT #258=(CScratch COMMENT (CWidget 3 (1 #3# 3) 1 2 0 0 #144# 0 100) COMMENT (LOGPEN 0 8 10053171) 8 1 0.0704623 1.9 COMMENT 0.082322 1) COMMENT #259=(CLabel (CWidget 0 (0 0) 1 2 0 0 #136# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 59 22 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Ava}I (6075)" "@N (@S)" 5 -4.47542 COMMENT 15.681 0 -10 5.18385 1.07863 #258#))))) COMMENT #260=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList)) COMMENT #261=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList))))) COMMENT (CSeqView 3 33 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers CDS complement(3..845) /vntifkey="4" /label=Dk\Sol CDS complement(5450..5905) /vntifkey="4" /label=f1\origin CDS 4542..5354 /vntifkey="4" /label=Kan promoter complement(917..933) /vntifkey="30" /label=T7\promoter rep_origin 3833..3833 /vntifkey="33" /label=pBR322\origin CDS 1320..2399 /vntifkey="4" /label=lacI CDS complement(6056..6073) /vntifkey="4" /label=His\Tag CDS complement(6095..6142) /vntifkey="4" /label=BSP\Sequence BASE COUNT 1408 a 1648 c 1652 g 1435 t ORIGIN 1 gatccggacg gaggaggctc ccatctcagg gtgaggggct caggcagccc ctcatggtac 61 acatggcatg tgtaattctg ctccttccca agaggcacca ccacagatgc ccacttctgg 121 aaggttccat cccctgcagg cctggtctcc acaagctcca tgtcctgggt cagctcctcc 181 ccattcaact gccaggtcag ggtgatgtca gcagggtaga agcccagggc ccagcacctc 241 agggtgactt caactttaga tctggggtga tgggtcacat gtgcctttgg ggaatctgtg 301 tgcagcagcg tcgcattccc gagctccagg tatctgcgga gccactccac gcacgtgccc 361 tccaggtagg cccggtctct ctctgcagca ccagcctgct cccacttgtg tttggtgatc 421 agcgccgcca tgtccgccgc cgtccacgtt ttcaggtctt cgttcagggc gatgtaatcg 481 cagccgtcgt atgcgaactg ctcgtacccg cggaggaggc gccagtccga ccccacgtca 541 cagccggaca gccgctggat cgtgtgagag ccaccctcgc tctggttgta gtagcggagc 601 agggtcctca ggtccactcg gaaactctgc tcattgccct tggcgatctg tgtctcccgt 661 tcccaatact ccggcccctc ctgctccatc caccgcaccc gcggctcatc tctcggattc 721 tccgcgtcgc tgtcgaagcg cacgaactcc gtgttgtcca cgtagccgac agagatgaac 781 cggggctctc cgaggccggg ccgggacacg acggtctcga aatatcttag tgaatgtggt 841 cccatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat 901 ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct 961 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta 1021 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg 1081 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt 1141 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt 1201 cctaatgcag gagtcgcata agggagagcg tcgagatccc ggacaccatc gaatggcgca 1261 aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg 1321 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt 1381 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg 1441 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt 1501 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg 1561 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac 1621 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg 1681 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca 1741 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt 1801 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc 1861 aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct 1921 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga 1981 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg 2041 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg 2101 ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat 2161 gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg 2221 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg 2281 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg 2341 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 2401 gagcgcaacg caattaatgt aagttagctc actcattagg caccgggatc tcgaccgatg 2461 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc 2521 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc 2581 tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt 2641 gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa 2701 cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccccacgggt gcgcatgatc 2761 gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat 2821 gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga 2881 gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca 2941 gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga 3001 acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc 3061 cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca 3121 tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg 3181 aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac 3241 atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac 3301 gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc 3361 agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 3421 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 3481 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg 3541 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatatgcg 3601 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc 3661 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 3721 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 3781 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 3841 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 3901 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 3961 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 4021 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 4081 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 4141 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 4201 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 4261 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 4321 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 4381 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 4441 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgaacaa 4501 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg 4561 aaacgtcttg ctctaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata 4621 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc 4681 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag 4741 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt 4801 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggg aaaacagcat 4861 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt 4921 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat 4981 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg 5041 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc 5101 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg 5161 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc 5221 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc 5281 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc 5341 tcgatgagtt tttctaagaa ttaattcatg agcggataca tatttgaatg tatttagaaa 5401 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga aattgtaaac 5461 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa 5521 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt 5581 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg 5641 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt 5701 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga 5761 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg 5821 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg 5881 cttaatgcgc cgctacaggg cgcgtcccat tcgccaatcc ggatatagtt cctcctttca 5941 gcaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctagt tattgctcag 6001 cggtggcagc agccaactca gcttcctttc gggctttgtt agcagccgga tctcagtggt 6061 ggtggtggtg gtgctcgagt gcggccgcaa gcttttaacg atgattccac accattttct 6121 gtgcatccag aatatgatgc agg //