LOCUS TCF\33-His 7300 bp DNA circular 9-JUN-2004 DEFINITION TCF 33 with His tag fused to BSP. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|340372474| COMMENT VNTDBDATE|340893539| COMMENT VNTNAME|TCF 33-His version II| COMMENT VNTAUTHORNAME|altman| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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"ggatcc" 2 0 1 1 0) COMMENT #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 2 132 0 5840 0) COMMENT #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0) COMMENT #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0) COMMENT #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 241 0 1586 0) COMMENT #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 1672 0) COMMENT #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 6023 0) COMMENT #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 6021 0) COMMENT #10=(CRSite (CStringList) "BglII" "agatct" 2 0 1 2124 0) COMMENT #11=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 2 861 0 6850 0) COMMENT #12=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 3296 0) COMMENT #13=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 11 0) COMMENT #14=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 2020 0) COMMENT #15=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 2013 0) COMMENT #16=(CRSite (CStringList) "SacI" "gagctc" 6 0 0) COMMENT #17=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 1316 0 2058 0) COMMENT #18=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 7250 0)) COMMENT (CObList COMMENT #19=(CFSignal (CObList) "2 Kb insert" 14 0 1 12 2011 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #20=(CFSignal (CObList) "DsRed1" 4 0 1 1331 2011 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #21=(CFSignal (CObList) "f1 origin" 4 0 1 6625 7080 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #22=(CFSignal (CObList) "Kan" 4 0 0 5717 6529 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #23=(CFSignal (CObList) "T7 promoter" 30 0 1 2092 2108 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #24=(CFSignal (CObList) "pBR322 origin" 33 0 0 5008 5008 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #25=(CFSignal (CObList) "lacI" 4 0 0 2495 3574 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #26=(CFSignal (CObList) "His Tag" 4 0 1 7231 7248 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #27=(CFSignal (CObList) "BSP" 21 0 1 7255 7299 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #28=(CFSignal (CObList) "T7 rev" 27 0 0 7160 7178 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #29=(CFSignal (CObList) "T7 fwd" 27 0 0 2088 2108 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #30=(CFSignal (CObList) "Mid sequence primer" 21 0 0 100 120 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) COMMENT (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #31=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #32=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) COMMENT "TCF 33-His version II" 2) 5 "" 0 4) COMMENT #33=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA Plasmid 'TCF 33-His version II'" 1) COMMENT #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "TCF 33 with His tag fused to BSP" 1) COMMENT 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(LOGFONT 47 17 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Ava}I (6021)" "@N (@S)" 5 -13.6139 COMMENT 5.22699 0 -10 5.1659 1.08218 #275#) COMMENT #277=(CScratch COMMENT (CWidget 3 (1 #1# 3) 1 2 0 0 #145# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 0.0438264 1.9 COMMENT 0.082322 1) COMMENT #278=(CLabel (CWidget 0 (0 0) 1 2 0 0 #137# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 47 17 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Ava}I (7250)" "@N (@S)" 5 -4.28037 COMMENT 19.1656 0 -10 5.20674 1.08218 #277#))))) COMMENT #279=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList)) COMMENT #280=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList))))) COMMENT (CSeqView 3 33 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers insertion_seq complement(12..2011) /vntifkey="14" /label=2\Kb\insert CDS complement(1331..2011) /vntifkey="4" /label=DsRed1 CDS complement(6625..7080) /vntifkey="4" /label=f1\origin CDS 5717..6529 /vntifkey="4" /label=Kan promoter complement(2092..2108) /vntifkey="30" /label=T7\promoter rep_origin 5008..5008 /vntifkey="33" /label=pBR322\origin CDS 2495..3574 /vntifkey="4" /label=lacI CDS complement(7231..7248) /vntifkey="4" /label=His\Tag misc_feature complement(7255..7299) /vntifkey="21" /label=BSP primer 7160..7178 /vntifkey="27" /label=T7\rev primer 2088..2108 /vntifkey="27" /label=T7\fwd misc_feature 100..120 /vntifkey="21" /label=Mid\sequence\primer /note="TCF33His_MidSeq_5p.ol" BASE COUNT 1692 a 1884 c 1949 g 1775 t ORIGIN 1 gatccggtac cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca 61 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc 121 tgtctcttga tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct 181 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc 241 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg 301 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg 361 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc 421 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagctggt 481 tctttccgcc tcaggactct tcctttttca atattattga agcatttatc agggttattg 541 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 601 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg 661 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 721 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 781 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 841 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 901 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 961 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 1021 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat 1081 ttacgcctta agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa 1141 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa 1201 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg 1261 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctaga 1321 gtcgcggccg ctacaggaac aggtggtggc ggccctcggt gcgctcgtac tgctccacga 1381 tggtgtagtc ctcgttgtgg gaggtgatgt ccagcttgga gtccacgtag tagtagccgg 1441 gcagctgcac gggcttcttg gccatgtaga tggacttgaa ctccaccagg tagtggccgc 1501 cgtccttcag cttcagggcc ttgtggatct cgcccttcag cacgccgtcg cgggggtaca 1561 ggcgctcggt ggaggcctcc cagcccatgg tcttcttctg cattacgggg ccgtcggagg 1621 ggaagttcac gccgatgaac ttcaccttgt agatgaagca gccgtcctgc agggaggagt 1681 cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc 1741 cctcggggaa ggacagcttc ttgtagtcgg ggatgtcggc ggggtgcttc acgtacacct 1801 tggagccgta ctggaactgg ggggacagga tgtcccaggc gaagggcagg gggccgccct 1861 tggtcacctt cagcttcacg gtgttgtggc cctcgtaggg gcggccctcg ccctcgccct 1921 cgatctcgaa ctcgtggccg ttcacggtgc cctccatgcg caccttgaag cgcatgaact 1981 ccttgatgac gttcttggag gagcgcacca tgctagccat atgtatatct ccttcttaaa 2041 gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag 2101 tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc 2161 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa 2221 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc 2281 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg 2341 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga 2401 gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata 2461 gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt 2521 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca 2581 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc 2641 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc 2701 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca 2761 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc 2821 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga 2881 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga 2941 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact 3001 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt 3061 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca 3121 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac 3181 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat 3241 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc 3301 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat 3361 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca 3421 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac 3481 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 3541 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt 3601 agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca 3661 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta 3721 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct 3781 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc 3841 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta 3901 tcgccggcat ggcggcccca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc 3961 taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt 4021 gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt 4081 tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga 4141 tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc 4201 gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt 4261 taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca 4321 tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg 4381 catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga 4441 cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg 4501 aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg 4561 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 4621 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg 4681 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc 4741 agagcagatt gtactgagag tgcaccatat atgcggtgtg aaataccgca cagatgcgta 4801 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 4861 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 4921 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 4981 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 5041 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 5101 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 5161 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 5221 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 5281 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 5341 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 5401 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 5461 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 5521 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 5581 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 5641 gaaaactcac gttaagggat tttggtcatg aacaataaaa ctgtctgctt acataaacag 5701 taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcta ggccgcgatt 5761 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca 5821 atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa 5881 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct 5941 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg 6001 gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga 6061 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc 6121 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg 6181 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt 6241 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac 6301 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat 6361 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg 6421 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa 6481 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aagaattaat 6541 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 6601 catttccccg aaaagtgcca cctgaaattg taaacgttaa tattttgtta aaattcgcgt 6661 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt 6721 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc 6781 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg 6841 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac 6901 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg 6961 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag 7021 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt 7081 cccattcgcc aatccggata tagttcctcc tttcagcaaa aaacccctca agacccgttt 7141 agaggcccca aggggttatg ctagttattg ctcagcggtg gcagcagcca actcagcttc 7201 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagacgatg 7261 attccacacc attttctgtg catccagaat atgatgcagg //