LOCUS TCF\33 7318 bp DNA circular 3-JUN-2004 DEFINITION 2 Kb fragment (containing DsRed1 gene) cut with NheI/KpnI, ligated into similarly cut JA 60. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDBDATE|340379143| COMMENT VNTNAME|TCF 33| COMMENT VNTAUTHORNAME|altman| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "TCF 33" 0 7318 COMMENT (CDBMol 0 0 1 2002 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList COMMENT #0=(CFragment 3 1 1 1886415973 0 "" 0 "" "" "" "" "" "" COMMENT "2 Kb insert primer extension" "" 0 0 1 2002 5318 1 9 2010 0 "" "" COMMENT "" "" "" "" (CObList) (CTerm 1 0 0 "NheI" "" 1 0 0 0 0 0 0 0 0 0) COMMENT (CTerm 1 1 0 "KpnI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) COMMENT #1=(CFragment 3 1 0 1037960580 0 "" 0 "" "" "" "" "" "" "JA 60" "" 0 0 COMMENT 1 5316 2 5317 283 10 0 "" "" "" "" "" "" (CObList) COMMENT (CTerm 1 0 0 "NheI" "" 1 0 0 0 0 0 0 0 0 0) COMMENT (CTerm 1 1 0 "KpnI" "" 1 0 0 0 0 0 0 0 0 0) 1 0)) (CObList)) COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 1 0 3 33 40 50 0 1 0 COMMENT (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" COMMENT "PstI" "SmaI" "XmaI" "BglII" "DraIII" "EcoRV" "KpnI" "NdeI" "NheI" COMMENT "SacI" "XbaI" "XhoI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 0 0 0 0 0) (CMolPar 0 0 0 0 0 1 7318 0 0 0 0 0 0 0 0) COMMENT (CStringList) (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList COMMENT #2=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 815 0 2750 0 3250 0) COMMENT #3=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 3 4010 0 5239 0 7050 0) COMMENT #4=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 5308 0) COMMENT #5=(CRSite (CStringList) "ClaI" "atcgat" 3 0 2 3829 0 5439 0) COMMENT #6=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0) COMMENT #7=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 5254 0) COMMENT #8=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 5548 0 6893 0) COMMENT #9=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 6979 0) COMMENT #10=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 4012 0) COMMENT #11=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 4010 0) COMMENT #12=(CRSite (CStringList) "BglII" "agatct" 2 0 1 113 0) COMMENT #13=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 2 4839 0 6168 0) COMMENT #14=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 1285 0) COMMENT #15=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 5318 0) COMMENT #16=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 9 0) COMMENT #17=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 2 0) COMMENT #18=(CRSite (CStringList) "SacI" "gagctc" 6 0 0) COMMENT #19=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 47 0 6623 0) COMMENT #20=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 5239 0)) COMMENT (CObList COMMENT #21=(CFSignal (CObList) "2 Kb insert" 14 0 1 5319 7318 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #22=(CFSignal (CObList) "DsRed1" 4 0 1 6638 7318 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #23=(CFSignal (CObList) "f1 origin" 4 0 1 4614 5069 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #24=(CFSignal (CObList) "Kan" 4 0 0 3706 4518 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #25=(CFSignal (CObList) "T7 promoter" 30 0 1 81 97 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #26=(CFSignal (CObList) "pBR322 origin" 33 0 0 2997 2997 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #27=(CFSignal (CObList) "lacI" 4 0 0 484 1563 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #28=(CFSignal (CObList) "His Tag" 4 0 1 5220 5237 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #29=(CFSignal (CObList) "BSP Sequence" 4 0 1 5259 5306 1 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #30=(CFSignal (CObList) "T7 Rev" 27 0 0 5149 5167 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #31=(CFSignal (CObList) "T7 fwd" 27 0 0 77 97 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) COMMENT (CObList) (CObList) COMMENT (CTextView 0 COMMENT #32=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #33=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "TCF 33" 2) 5 "" 0 COMMENT 4) COMMENT #34=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA Plasmid 'TCF 33'" 1) COMMENT #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "2 Kb fragment (containing DsRed1 gene) cut with NheI/KpnI, ligated into similarly cut JA 60" COMMENT 1) COMMENT #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Currently local object. Original author: UNKNOWN" 1) COMMENT #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Creation Date: ? 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COMMENT "parent position: from 9 to 2010" 1) COMMENT #49=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "original length: 2002" 1) COMMENT #50=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "molecule position: from 5318 to 1" 1) COMMENT #51=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #52=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " NheI site #1" 1))) "Left Terminus") COMMENT #53=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #54=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " KpnI site #1" 1))) "Right Terminus"))) COMMENT "#1: FRAGMENT of 2 Kb insert primer extension (Complementary)") COMMENT #55=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (5 #1# 0) 1 2 1 0 195) COMMENT (CObjectList COMMENT #56=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "parent position: from 283 to 10" 1) COMMENT #57=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "original length: 5316" 1) COMMENT #58=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "molecule position: from 2 to 5317" 1) COMMENT #59=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #60=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " NheI site #1" 1))) "Left Terminus") COMMENT #61=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #62=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " KpnI site #1" 1))) "Right Terminus"))) COMMENT "#2: FRAGMENT of JA 60 "))) COMMENT "Component Fragments") COMMENT #63=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #64=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #65=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 484 (3 #27# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #66=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 484 End: 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#75=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 6638 (3 #22# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #76=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 6638 End: 7318 (Complementary)" COMMENT 1))) "DsRed1"))) "CDS (6 total)") COMMENT #77=(CFolderPar COMMENT (CGroupPar (CParagraph 14 (7 14 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #78=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5319 (3 #21# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #79=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5319 End: 7318 (Complementary)" COMMENT 1))) "2 Kb insert"))) COMMENT "Insertion (1 total)") COMMENT #80=(CFolderPar COMMENT (CGroupPar (CParagraph 27 (7 27 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #81=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 77 (3 #31# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #82=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 77 End: 97 " 1))) "T7 fwd") COMMENT #83=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5149 (3 #30# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #84=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5149 End: 5167" 1))) "T7 Rev"))) COMMENT "Primer (2 total)") COMMENT #85=(CFolderPar COMMENT (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #86=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 81 (3 #25# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #87=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 81 End: 97 (Complementary)" COMMENT 1))) "T7 promoter"))) COMMENT "Promoter Prokaryotic (1 total)") COMMENT #88=(CFolderPar COMMENT (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #89=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2997 (3 #26# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #90=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2997 End: 2997" 1))) COMMENT "pBR322 origin"))) COMMENT "Replication Origin (1 total)"))) 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COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 47 17 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Ava}I (5239)" "@N (@S)" 5 -14.5218 COMMENT 1.74233 0 -10 5.19427 1.07959 #291#) COMMENT #293=(CScratch COMMENT (CWidget 3 (1 #3# 3) 1 2 0 0 #161# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 0.230594 1.9 COMMENT 0.082322 1) COMMENT #294=(CLabel (CWidget 0 (0 0) 1 2 0 0 #153# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 47 17 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Ava}I (7050)" "@N (@S)" 5 -6.59266 COMMENT 24.3926 0 -10 5.25538 1.07959 #293#))))) COMMENT #295=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList)) COMMENT #296=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList))))) COMMENT (CSeqView 3 33 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers insertion_seq complement(5319..7318) /vntifkey="14" /label=2\Kb\insert CDS complement(6638..7318) /vntifkey="4" /label=DsRed1 CDS complement(4614..5069) /vntifkey="4" /label=f1\origin CDS 3706..4518 /vntifkey="4" /label=Kan promoter complement(81..97) /vntifkey="30" /label=T7\promoter rep_origin 2997..2997 /vntifkey="33" /label=pBR322\origin CDS 484..1563 /vntifkey="4" /label=lacI CDS complement(5220..5237) /vntifkey="4" /label=His\Tag CDS complement(5259..5306) /vntifkey="4" /label=BSP\Sequence primer 5149..5167 /vntifkey="27" /label=T7\Rev primer 77..97 /vntifkey="27" /label=T7\fwd BASE COUNT 1695 a 1889 c 1954 g 1780 t ORIGIN 1 gctagccata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg 61 ttatccgctc acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatctcga 121 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg 181 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg 241 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct 301 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct 361 gcttcctaat gcaggagtcg cataagggag agcgtcgaga tcccggacac catcgaatgg 421 cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg 481 aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc 541 gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa 601 gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa 661 cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt 721 gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta 781 gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc 841 agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc 901 tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt 961 attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac 1021 cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct 1081 ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac 1141 tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc 1201 actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag 1261 tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc 1321 tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc 1381 agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg 1441 cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc 1501 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 1561 cagtgagcgc aacgcaatta atgtaagtta gctcactcat taggcaccgg gatctcgacc 1621 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat 1681 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc 1741 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc 1801 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac 1861 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccccac gggtgcgcat 1921 gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca 1981 gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac 2041 ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa 2101 gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg 2161 tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg 2221 gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg 2281 ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc 2341 catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct 2401 taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct 2461 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta 2521 ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 2581 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 2641 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg 2701 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatata 2761 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg 2821 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 2881 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 2941 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 3001 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 3061 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 3121 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 3181 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 3241 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 3301 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 3361 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 3421 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 3481 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 3541 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 3601 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 3661 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa 3721 cgggaaacgt cttgctctag gccgcgatta aattccaaca tggatgctga tttatatggg 3781 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg 3841 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt 3901 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag 3961 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca 4021 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca 4081 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc 4141 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat 4201 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt 4261 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt 4321 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga 4381 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa 4441 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg 4501 atgctcgatg agtttttcta agaattaatt catgagcgga tacatatttg aatgtattta 4561 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaaattgt 4621 aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa 4681 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt 4741 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa 4801 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag 4861 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt 4921 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg 4981 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc 5041 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca atccggatat agttcctcct 5101 ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc tagttattgc 5161 tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc cggatctcag 5221 tggtggtggt ggtggtgctc gagtgcggcc gcaagctttt aacgatgatt ccacaccatt 5281 ttctgtgcat ccagaatatg atgcagggat ccggtaccag ccgattgtct gttgtgccca 5341 gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg 5401 ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc 5461 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct 5521 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag 5581 ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca 5641 tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact 5701 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc 5761 ctaactgaca cacattccac agctggttct ttccgcctca ggactcttcc tttttcaata 5821 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 5881 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc 5941 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 6001 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 6061 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 6121 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 6181 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 6241 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 6301 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 6361 ttttaacaaa atattaacgc ttacaattta cgccttaaga tacattgatg agtttggaca 6421 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc 6481 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt 6541 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa 6601 atgtggtatg gctgattatg atctagagtc gcggccgcta caggaacagg tggtggcggc 6661 cctcggtgcg ctcgtactgc tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca 6721 gcttggagtc cacgtagtag tagccgggca gctgcacggg cttcttggcc atgtagatgg 6781 acttgaactc caccaggtag tggccgccgt ccttcagctt cagggccttg tggatctcgc 6841 ccttcagcac gccgtcgcgg gggtacaggc gctcggtgga ggcctcccag cccatggtct 6901 tcttctgcat tacggggccg tcggagggga agttcacgcc gatgaacttc accttgtaga 6961 tgaagcagcc gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga 7021 agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcttg tagtcgggga 7081 tgtcggcggg gtgcttcacg tacaccttgg agccgtactg gaactggggg gacaggatgt 7141 cccaggcgaa gggcaggggg ccgcccttgg tcaccttcag cttcacggtg ttgtggccct 7201 cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggtgccct 7261 ccatgcgcac cttgaagcgc atgaactcct tgatgacgtt cttggaggag cgcaccat //