LOCUS pTCF\48 6152 bp DNA circular 23-SEP-2003 DEFINITION HLA A1101 cut with NheI/BamHI, ligated into similarly cut TCF 33. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|311862444| COMMENT VNTDBDATE|314470561| COMMENT VNTNAME|pTCF 48| COMMENT VNTAUTHORNAME|altman| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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COMMENT #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 4674 0) COMMENT #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0) COMMENT #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 6099 0) COMMENT #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 0) COMMENT #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 142 0 311 0) COMMENT #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 4857 0) COMMENT #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 4855 0) COMMENT #10=(CRSite (CStringList) "BglII" "agatct" 2 0 1 958 0) COMMENT #11=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 1 5684 0) COMMENT #12=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 2130 0) COMMENT #13=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 511 0) COMMENT #14=(CRSite (CStringList) "NdeI" "catatg" 3 0 2 281 0 854 0) COMMENT #15=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 847 0) COMMENT #16=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 161 0) COMMENT #17=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 892 0) COMMENT #18=(CRSite (CStringList) "XhoI" 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-5.22699 0 -10 5.89443 1.07399 #247#))))) COMMENT #249=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList)) COMMENT #250=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList))))) COMMENT (CSeqView 3 33 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers CDS complement(6..845) /vntifkey="4" /label=HLA\A1101 /note="HLA A1101 " CDS complement(5459..5914) /vntifkey="4" /label=f1\origin CDS 4551..5363 /vntifkey="4" /label=Kan promoter complement(926..942) /vntifkey="30" /label=T7\promoter rep_origin 3842..3842 /vntifkey="33" /label=pBR322\origin CDS 1329..2408 /vntifkey="4" /label=lacI CDS complement(6065..6082) /vntifkey="4" /label=His\Tag CDS complement(6104..6151) /vntifkey="4" /label=BSP\Sequence BASE COUNT 1389 a 1659 c 1674 g 1430 t ORIGIN 1 gatccgggct gggaagacag ctcccatctc agggtgaggg gcttgggcag accctcatgc 61 tgcacatggc aggtgtatct ctgctcctct ccagaaggca ccaccacagc cgcccacttc 121 tggaaggttc catcccctgc aggcctggtc tccacgagct ccgtgtcctg ggtctggtcc 181 tccccatccc gctgccaggt cagtgtgatc tccgcagggt agaagcccag ggcccagcac 241 ctcagggtgg cctcatggtc agagatgggg tggtgggtca tatgtgtctt gggggggtcc 301 gtgcgctgca gcgtctcctt cccgttctcc aggtatctgc ggagccactc cacgcaccgg 361 ccctccaggt aggctctctg ctgctccgcc gcatgggccg cctcccactt gcgcttggtg 421 atctgagctg ccatgtccgc cgcggtccaa gagcgcaggt cctcgttcag ggcgatgtaa 481 tccttgccgt cgtaggcgtc ctgccggtac ccgcggagga agcgcccgtc cggccccacg 541 tcgcagccat acattatctg gatggtgtga gaaccgtcct cgctctggtt gtagtagccg 601 cgcagggtcc ccaggtccac tcggtcagtc tgtgactggg ccttcacatt ccgtgtctcc 661 tggtcccaat actccggccc ctcctgctct atccacggcg cccgcggctc catcctctgg 721 ctcgcggcgt cgctgtcgaa ccgcacgaac tgcgtgtcgt ccacgtagcc cacggcgatg 781 aagcggggct ccccgcggcc gggccgggac acggaggtgt agaaatatct catagaatga 841 gaaccgctag ccatatgtat atctccttct taaagttaaa caaaattatt tctagagggg 901 aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat 961 ctcgatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc 1021 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat 1081 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc 1141 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact 1201 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgagatcccg gacaccatcg 1261 aatggcgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg 1321 tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc 1381 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag 1441 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg 1501 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc 1561 aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga 1621 tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac 1681 gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag 1741 ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca 1801 gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg 1861 gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc 1921 tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag 1981 gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg 2041 ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta 2101 ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag 2161 acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc 2221 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc 2281 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct 2341 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 2401 gcgggcagtg agcgcaacgc aattaatgta agttagctca ctcattaggc accgggatct 2461 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg 2521 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg 2581 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc 2641 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc 2701 gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cccacgggtg 2761 cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt 2821 tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct 2881 gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg 2941 cggaagtcag cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta 3001 ccctgtggaa cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt 3061 ctctggtccc gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg 3121 gcatgttcat catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt 3181 acccccatga acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc 3241 gcccttaaca tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac 3301 gagctggacg cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag 3361 ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag 3421 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag 3481 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat 3541 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc 3601 atatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc 3661 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 3721 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 3781 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 3841 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 3901 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 3961 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 4021 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 4081 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 4141 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 4201 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 4261 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 4321 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 4381 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 4441 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 4501 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata 4561 ttcaacggga aacgtcttgc tctaggccgc gattaaattc caacatggat gctgatttat 4621 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt 4681 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg 4741 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca 4801 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggga 4861 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc 4921 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg 4981 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga 5041 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata 5101 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc 5161 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag 5221 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac 5281 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc 5341 atttgatgct cgatgagttt ttctaagaat taattcatga gcggatacat atttgaatgt 5401 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgaa 5461 attgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt 5521 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata 5581 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac 5641 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa 5701 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc 5761 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg 5821 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca 5881 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccaatccg gatatagttc 5941 ctcctttcag caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctagtt 6001 attgctcagc ggtggcagca gccaactcag cttcctttcg ggctttgtta gcagccggat 6061 ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttttaacga tgattccaca 6121 ccattttctg tgcatccaga atatgatgca gg //