LOCUS TCF\5 6152 bp DNA circular 19-FEB-2003 DEFINITION HLA A0101 Soluble fragment cut with NheI and BamHI, ligated into similarly cut JA 60. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDBDATE|294771249| COMMENT VNTNAME|TCF 5| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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0 (0 0) 1 2 0 0 #121# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 53 20 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Apa}LI (3250)" "@N (@S)" 5 -5.92335 COMMENT -12.1963 0 -10 5.77031 1.08975 #248#))))) COMMENT #250=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList)) COMMENT #251=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList))))) COMMENT (CSeqView 3 33 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers CDS complement(5313..6152) /vntifkey="4" /label=HLA\A*0101\Sol CDS complement(4614..5069) /vntifkey="4" /label=f1\origin CDS 3706..4518 /vntifkey="4" /label=Kan promoter complement(81..97) /vntifkey="30" /label=T7\promoter rep_origin 2997..2997 /vntifkey="33" /label=pBR322\origin CDS 484..1563 /vntifkey="4" /label=lacI terminator 5106..5148 /vntifkey="43" /label=T7\terminator CDS complement(5259..5312) /vntifkey="4" /label=BSP\sequence BASE COUNT 1390 a 1657 c 1674 g 1431 t ORIGIN 1 gctagccata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg 61 ttatccgctc acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatctcga 121 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg 181 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg 241 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct 301 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct 361 gcttcctaat gcaggagtcg cataagggag agcgtcgaga tcccggacac catcgaatgg 421 cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg 481 aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc 541 gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa 601 gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa 661 cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt 721 gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta 781 gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc 841 agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc 901 tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt 961 attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac 1021 cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct 1081 ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac 1141 tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc 1201 actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag 1261 tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc 1321 tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc 1381 agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg 1441 cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc 1501 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 1561 cagtgagcgc aacgcaatta atgtaagtta gctcactcat taggcaccgg gatctcgacc 1621 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat 1681 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc 1741 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc 1801 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac 1861 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccccac gggtgcgcat 1921 gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca 1981 gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac 2041 ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa 2101 gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg 2161 tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg 2221 gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg 2281 ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc 2341 catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct 2401 taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct 2461 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta 2521 ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 2581 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 2641 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg 2701 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatata 2761 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg 2821 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 2881 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 2941 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 3001 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 3061 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 3121 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 3181 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 3241 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 3301 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 3361 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 3421 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 3481 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 3541 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 3601 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 3661 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa 3721 cgggaaacgt cttgctctag gccgcgatta aattccaaca tggatgctga tttatatggg 3781 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg 3841 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt 3901 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag 3961 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca 4021 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca 4081 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc 4141 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat 4201 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt 4261 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt 4321 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga 4381 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa 4441 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg 4501 atgctcgatg agtttttcta agaattaatt catgagcgga tacatatttg aatgtattta 4561 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaaattgt 4621 aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa 4681 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt 4741 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa 4801 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag 4861 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt 4921 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg 4981 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc 5041 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca atccggatat agttcctcct 5101 ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc tagttattgc 5161 tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc cggatctcag 5221 tggtggtggt ggtggtgctc gagtgcggcc gcaagctttt aacgatgatt ccacaccatt 5281 ttctgtgcat ccagaatatg atgcagggat ccgggctggg aagacagctc ccatctcagg 5341 gtgaggggct tgggcagacc ctcatgctgc acatggcagg tgtatctctg ctcctctcca 5401 gaaggcacca ccacagccgc ccacttctgg aaggttccat cccctgcagg cctggtctcc 5461 acgagctccg tgtcctgggt ctggtcctcc ccatcccgct gccaggtcag tgtgatctcc 5521 gcagggtaga agcccagggc ccagcacctc agggtggcct catggtcaga gatggggtgg 5581 tgggtcatat gtgtcttggg ggggtccgtg cgctgcagcg tctccttccc gttctccagg 5641 tatctgcgga gcccgtccac gcaccggccc tccaggtaga ctctccgctg ctccgccgca 5701 tggaccgcct cccacttgcg cttggtgatc tgagctgcca tgtccgccgc ggtccaagag 5761 cgcaggtcct cgttcagggc gatgtaatcc ttgccgtcgt aggcgtcctg ccggtacccg 5821 cggaggaagc gcccgtccgg ccccacgtcg cagccataca ttatctggat ggtgtgagaa 5881 ccgtcctcgc tctggttgta gtagccgcgc agggtcccca ggttcgctcg gtcagtctgt 5941 gagtgggcct tcatattccg tgtctcctgg tcccaatact ccggcccctc ctgctctatc 6001 cacggcgccc gcggctccat cttctggctc gcggcgtcgc tgtcgaaccg cacgaactgc 6061 gtgtcgtcca cgtagcccac ggcgatgaag cggggctccc cgcggccggg ccgggacacg 6121 gatgtgaaga aatacctcat agaatgagaa cc //