LOCUS TCF\9 6152 bp DNA circular 19-FEB-2003 DEFINITION HLA A0205 cut with NheI/BamHI, ligated into similarly cut JA 60. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDBDATE|294771250| COMMENT VNTNAME|TCF 9| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 51 19 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Apa}LI (2749)" "@N (@S)" 5 7.60245 COMMENT -12.1963 0 -10 5.74706 1.08576 #249#) COMMENT #251=(CScratch COMMENT (CWidget 3 (1 #0# 3) 1 2 0 0 #130# 0 100) COMMENT (LOGPEN 0 8 10053171) 8 1 2.9612 1.9 0.082322 COMMENT 1) COMMENT #252=(CLabel (CWidget 0 (0 0) 1 2 0 0 #122# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 51 19 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Apa}LI (3249)" "@N (@S)" 5 -5.90262 COMMENT -12.1963 0 -10 5.80322 1.08576 #251#))))) COMMENT #253=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList)) COMMENT #254=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList))))) COMMENT (CSeqView 3 33 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers CDS complement(4613..5068) /vntifkey="4" /label=f1\origin CDS 3705..4517 /vntifkey="4" /label=Kan promoter complement(80..96) /vntifkey="30" /label=T7\promoter rep_origin 2996..2996 /vntifkey="33" /label=pBR322\origin CDS 483..1562 /vntifkey="4" /label=lacI terminator 5105..5147 /vntifkey="43" /label=T7\terminator CDS complement(5312..6151) /vntifkey="4" /label=A*0205\Sol CDS complement(5258..5311) /vntifkey="4" /label=BSP\sequence BASE COUNT 1394 a 1657 c 1670 g 1431 t ORIGIN 1 ctagccatat gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt 61 tatccgctca caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat 121 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc 181 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc 241 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc 301 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg 361 cttcctaatg caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc 421 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga 481 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg 541 tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag 601 cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac 661 agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg 721 tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag 781 aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca 841 gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct 901 gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta 961 ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc 1021 agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg 1081 gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact 1141 ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca 1201 ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt 1261 ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct 1321 catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca 1381 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc 1441 ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc 1501 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 1561 agtgagcgca acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg 1621 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc 1681 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg 1741 ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg 1801 cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc 1861 aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccccacg ggtgcgcatg 1921 atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag 1981 aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc 2041 tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag 2101 tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt 2161 ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg 2221 tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt 2281 tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc 2341 atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt 2401 aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg 2461 gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac 2521 cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg 2581 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 2641 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga 2701 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatat 2761 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc 2821 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 2881 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 2941 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 3001 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 3061 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 3121 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 3181 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 3241 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 3301 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 3361 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 3421 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 3481 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 3541 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 3601 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgaa 3661 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac 3721 gggaaacgtc ttgctctagg ccgcgattaa attccaacat ggatgctgat ttatatgggt 3781 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga 3841 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta 3901 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc 3961 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag 4021 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag 4081 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg 4141 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt 4201 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt 4261 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt 4321 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat 4381 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac 4441 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga 4501 tgctcgatga gtttttctaa gaattaattc atgagcggat acatatttga atgtatttag 4561 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaaattgta 4621 aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac 4681 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg 4741 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa 4801 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt 4861 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt 4921 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga 4981 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc 5041 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccaa tccggatata gttcctcctt 5101 tcagcaaaaa acccctcaag acccgtttag aggccccaag gggttatgct agttattgct 5161 cagcggtggc agcagccaac tcagcttcct ttcgggcttt gttagcagcc ggatctcagt 5221 ggtggtggtg gtggtgctcg agtgcggccg caagctttta acgatgattc cacaccattt 5281 tctgtgcatc cagaatatga tgcagggatc cgggctggga agacggctcc catctcaggg 5341 tgaggggctt gggcaaaccc tcatgctgca catggcaggt gtatctctgc tcctgtccag 5401 aaggcaccac cacagccgcc cacttctgga aggttccatc ccctgcaggc ctggtctcca 5461 cgagctccgt gtcctgggtc tggtcctccc catcccgctg ccaggtcagt gtgatctccg 5521 cagggtagaa gctcagggcc cagcacctca gggtggcttc atggtcagag acagcgtggt 5581 gagtcatatg cgttttgggg gcgtccgtgc gctgcagcgt ctccttcccg ttctccaggt 5641 atctgcggag ccactccacg cacgtgccct ccaggtaggc tctccactgc tccgccacat 5701 gggccgcctc ccacttgtgc ttggtggtct gagctgccat gtccgccgcg gtccaagagc 5761 gcaggtcctc tttcagggcg atgtaatcct tgccgtcgta ggcgtactgg tggtacccgc 5821 gcaggaagcg ccagtccgac cccacgtcgc agccatacat cctctggagg gtgtgagaac 5881 cggcctcgct ctggttgtag tagccgcgca gggtccccag gtccactcgg tgagtctgtg 5941 agtgggcctt cactttccgt gtctccccgt cccaatactc cggaccctcc tgctctatcc 6001 acggcgcccg cggctccatc ctccggctcg cggcgtcgct gtcgaaccgc acgaactgcg 6061 tgtcgtccac gtagcccact gcgatgaagc ggggctcccc gcggccgggc cgggacacgg 6121 aggtgtagaa atatctcata gaatgagaac cg //