Flow Cytometry Protocols and Tech Notes
Protocols on this site
Antibodies and Labels
- Comments on Labeling Antibodies
- Fluorescent Dye Groups
- Differences between fluorescein derivatives
- Choosing FL3 reagents for the FACSCalibur
- Antibody stocks
- Titering antibodies
- Titration of human mAbs using whole blood samples in deep well plates
- Antibody storage
- Comments on BSA
- Whole blood staining
Live/Dead discrimination
- Live/Dead Discrimination with EMA (protocol from Steve DeRosa)
- Live/Dead Fixable Cell Stain from Invitrogen/Molecular Probes (PDF)
Instrumentation
- Instrument Configurations on the Aria and LSR-II
- Development of polychromatic flow cytometry on the FACS Aria at the Emory Vaccine Center
- Using the MAS
Software and data analysis
- Annotating FCS Files
- Caution in overlay gating
- Preparation of publication/presentation quality figures of FlowJo batch layouts using FlowJo and Canvas
External Documents
Compensation Beads Technical Data Sheet (pdf) | BD CompBeads Home Page | CompBeads Technical Applications Sheet
FMO - Fluoresence Minus One (Roederer Cytometry paper)